| Paper: | WP-P2.1 |
| Session: | Biomedical Imaging V: Molecular & Cellular Bioimaging / Segmentation |
| Time: | Wednesday, September 19, 14:30 - 17:10 |
| Presentation: |
Poster
|
| Title: |
3D FLUORESCENT SPOTS DETECTION IN LINE-SCANNING CONFOCAL MICROSCOPY |
| Authors: |
Elodie Dusch; Institut Pasteur Korea | | |
| | Nicole Vincent; Université René Descartes Paris 5 | | |
| | Auguste Genovesio; Institut Pasteur Korea | | |
| Abstract: |
This paper describes a method to detect fluorescent PSF-like spots in 3d line-scanning confocal microscopy. We suppose that fluorescent spots are small enough to be characterized by the Point Spread function (PSF) of the microscope. To localize spots, we use a derivative expression of the image previously convolved by a Gaussian approximating the PSF. The algorithm validation is made on 3D synthetic images with fluorescent background structures and on a 3D fluorescent biological image. |
